Human ARIH2 Recombinant Protein (N-His) (HD428012)

Name: Human ARIH2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD428012
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HD428012
Description	Recombinant Human ARIH2 Protein, N-His (HD428012) expressed in E. coli, spanning Thr62-Thr493. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O95376
Protein length	Thr62-Thr493
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	53.19 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ARI-2, ARI2, ARIH2, E3 ubiquitin-protein ligase ARIH2, EC:2.3.2.31, Protein ariadne-2 homolog, TRIAD1, Triad1 protein
Background	E3 ubiquitin-protein ligase ARIH2 is a ~57 kDa protein. E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets. The initial ubiquitin is then elongated. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex. Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB. 1. Marteijn, JA. et al. (2005) Blood 106, 4114-23. PMID: 16118314 2. Marteijn, JA. et al. (2007) Blood 110, 3128-35. PMID: 17646546 3. Marteijn, JA. et al. (2009) Leukemia 23, 1480-9. PMID: 19340006 4. Kelsall, IR. et al. (2013) The EMBO journal 32, 2848-60. PMID: 24076655 5. Lumpkin, RJ. et al. (2021) Molecular & cellular proteomics : MCP 20, 100019. PMID: 33268465 6. Kostrhon, S. et al. (2021) Nature chemical biology 17, 1075-1083. PMID: 34518685 7. Xu, P. et al. (2024) Nature 627, 873-879. PMID: 38418882
Note	For research use only

Images

References

Datasheet

Human ARIH2 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Human ARIH2 Recombinant Protein (N-His) (HD428012)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us