Anti-ARIH2 Polyclonal Antibody (HD428014)

Name: Anti-ARIH2 Polyclonal Antibody
Brand: abinScience
SKU: HD428014
Price: 96 USD
Availability: InStock

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SDS

Overview

Catalog No.

HD428014

Description

Anti-ARIH2 Polyclonal Antibody (HD428014) is a rabbit polyclonal antibody detecting ARIH2 in ELISA, IHC, WB. Suitable for Mouse and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ARIH2 (Thr62-Thr493).

Target

ARI-2, ARI2, ARIH2, E3 ubiquitin-protein ligase ARIH2, EC:2.3.2.31, Protein ariadne-2 homolog, TRIAD1, Triad1 protein

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O95376

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

O95376

Background

E3 ubiquitin-protein ligase ARIH2 is a ~57 kDa protein. E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets. The initial ubiquitin is then elongated. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex. Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB.

1. Marteijn, JA. et al. (2005) Blood 106, 4114-23. PMID: 16118314

2. Marteijn, JA. et al. (2007) Blood 110, 3128-35. PMID: 17646546

3. Marteijn, JA. et al. (2009) Leukemia 23, 1480-9. PMID: 19340006

4. Kelsall, IR. et al. (2013) The EMBO journal 32, 2848-60. PMID: 24076655

5. Lumpkin, RJ. et al. (2021) Molecular & cellular proteomics : MCP 20, 100019. PMID: 33268465

6. Kostrhon, S. et al. (2021) Nature chemical biology 17, 1075-1083. PMID: 34518685

7. Xu, P. et al. (2024) Nature 627, 873-879. PMID: 38418882

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-ARIH2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: 293T cell lysate
Lane 5: Hela cell lysate

References

Datasheet

Anti-ARIH2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-ARIH2 Polyclonal Antibody (HD428014)

Overview

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References

Datasheet

SDS

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