Anti-ARIH2 Polyclonal Antibody (HD428014)

Name: Anti-ARIH2 Polyclonal Antibody
Brand: abinScience
SKU: HD428014
Price: 96 USD
Availability: InStock

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Spécification :

20ug
50ug
100ug

Nombre :

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HD428014

Description

Anti-ARIH2 Polyclonal Antibody (HD428014) is a rabbit polyclonal antibody detecting ARIH2 in ELISA, IHC, WB. Suitable for Mouse and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ARIH2 (Thr62-Thr493).

Cible

ARI-2, ARI2, ARIH2, E3 ubiquitin-protein ligase ARIH2, EC:2.3.2.31, Protein ariadne-2 homolog, TRIAD1, Triad1 protein

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

O95376

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Noms alternatifs

O95376

Contexte

E3 ubiquitin-protein ligase ARIH2 is a ~57 kDa protein. E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets. The initial ubiquitin is then elongated. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex. Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB.

1. Marteijn, JA. et al. (2005) Blood 106, 4114-23. PMID: 16118314

2. Marteijn, JA. et al. (2007) Blood 110, 3128-35. PMID: 17646546

3. Marteijn, JA. et al. (2009) Leukemia 23, 1480-9. PMID: 19340006

4. Kelsall, IR. et al. (2013) The EMBO journal 32, 2848-60. PMID: 24076655

5. Lumpkin, RJ. et al. (2021) Molecular & cellular proteomics : MCP 20, 100019. PMID: 33268465

6. Kostrhon, S. et al. (2021) Nature chemical biology 17, 1075-1083. PMID: 34518685

7. Xu, P. et al. (2024) Nature 627, 873-879. PMID: 38418882

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-ARIH2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: 293T cell lysate
Lane 5: Hela cell lysate

Reférences

Fiche technique

Anti-ARIH2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-ARIH2 Polyclonal Antibody (HD428014)

Aperçu

Images

Reférences

Fiche technique

SDS