Anti-ARIH2 Polyclonal Antibody (HD428014)

Name: Anti-ARIH2 Polyclonal Antibody
Brand: abinScience
SKU: HD428014
Price: 96 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD): --

Спецификация:

20ug
50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HD428014

Описание

Anti-ARIH2 Polyclonal Antibody (HD428014) is a rabbit polyclonal antibody detecting ARIH2 in ELISA, IHC, WB. Suitable for Mouse and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human ARIH2 (Thr62-Thr493).

Цель

ARI-2, ARI2, ARIH2, E3 ubiquitin-protein ligase ARIH2, EC:2.3.2.31, Protein ariadne-2 homolog, TRIAD1, Triad1 protein

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

O95376

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Альтернативные названия

O95376

Фон

E3 ubiquitin-protein ligase ARIH2 is a ~57 kDa protein. E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets. The initial ubiquitin is then elongated. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex. Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB.

1. Marteijn, JA. et al. (2005) Blood 106, 4114-23. PMID: 16118314

2. Marteijn, JA. et al. (2007) Blood 110, 3128-35. PMID: 17646546

3. Marteijn, JA. et al. (2009) Leukemia 23, 1480-9. PMID: 19340006

4. Kelsall, IR. et al. (2013) The EMBO journal 32, 2848-60. PMID: 24076655

5. Lumpkin, RJ. et al. (2021) Molecular & cellular proteomics : MCP 20, 100019. PMID: 33268465

6. Kostrhon, S. et al. (2021) Nature chemical biology 17, 1075-1083. PMID: 34518685

7. Xu, P. et al. (2024) Nature 627, 873-879. PMID: 38418882

Примечание

For research use only

Изображения

Western blot

Western blot analysis was performed using anti-ARIH2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: 293T cell lysate
Lane 5: Hela cell lysate

Ссылки

Даташит

Anti-ARIH2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-ARIH2 Polyclonal Antibody (HD428014)

Обзор

Изображения

Ссылки

Даташит

SDS