Anti-ASFV g5Rp/D250R Polyclonal Antibody (VK490014)

Name: Anti-ASFV g5Rp/D250R Polyclonal Antibody
Brand: abinScience
SKU: VK490014
Price: 168 USD
Availability: InStock

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Изображения
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Даташит
SDS

Обзор

Каталожный номер

VK490014

Описание

Anti-ASFV g5Rp/D250R Polyclonal Antibody (VK490014) is a rabbit polyclonal antibody detecting D250R in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

African swine fever virus (ASFV)

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant ASFV g5Rp/D250R (Met1-His250).

Цель

mRNA-decapping protein g5R, g5Rp, 3.1.3.-, ASFV-DP, Diphosphoinositol polyphosphate phosphohydrolase, DIPP, 3.6.1.52, Ba71V-102, D250R

Очистка

Purified by antigen affinity column.

Номер доступа

P32092

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

mRNA-decapping protein g5R (g5Rp/D250R) is a ~29 kDa protein. Decapping enzyme required for the removal of the 5'-end m7GpppN cap tethered to viral and host mRNAs to allow their decay in cells. May therefore accelerate viral and cellular mRNA turnover to eliminate competing host mRNAs and allow stage-specific synthesis of viral proteins. Acceleration of the turnover of cellular transcripts may even promote the shutoff of host protein synthesis. In addition to the mRNA cap, g5R also efficiently hydrolyzes diphosphoinositol polyphosphates. Down-regulation of the level of PP-InsP5 (diphosphoinositol pentakisphosphate) may play a role in viral manipulation of the cellular secretory pathway, a step necessary for the formation of virions.

1. Parrish, S. et al. (2009) Virology 393, 177-82. PMID: 19695654

2. Quintas, A. et al. (2017) Journal of virology 91. PMID: 29021398

3. Cartwright, JL. et al. (2002) Journal of virology 76, 1415-21. PMID: 11773415

Примечание

For research use only.

Изображения

Western blot

Lane 1: recombinant ASFV g5Rp/D250R (Catalog No: VK490012)

Ссылки

Даташит

Anti-ASFV g5Rp/D250R Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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