ASFV g5Rp/D250R Recombinant Protein (N-His) (VK490012)

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Catalog No.	VK490012
Description	Recombinant ASFV g5Rp/D250R Protein, N-His (VK490012) expressed in E. coli, spanning Met1-His250. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P32092
Protein length	Met1-His250
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	African swine fever virus (ASFV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	mRNA-decapping protein g5R, g5Rp, 3.1.3.-, ASFV-DP, Diphosphoinositol polyphosphate phosphohydrolase, DIPP, 3.6.1.52, Ba71V-102, D250R
Background	mRNA-decapping protein g5R (g5Rp/D250R) is a ~29 kDa protein. Decapping enzyme required for the removal of the 5'-end m7GpppN cap tethered to viral and host mRNAs to allow their decay in cells. May therefore accelerate viral and cellular mRNA turnover to eliminate competing host mRNAs and allow stage-specific synthesis of viral proteins. Acceleration of the turnover of cellular transcripts may even promote the shutoff of host protein synthesis. In addition to the mRNA cap, g5R also efficiently hydrolyzes diphosphoinositol polyphosphates. Down-regulation of the level of PP-InsP5 (diphosphoinositol pentakisphosphate) may play a role in viral manipulation of the cellular secretory pathway, a step necessary for the formation of virions. 1. Parrish, S. et al. (2009) Virology 393, 177-82. PMID: 19695654 2. Quintas, A. et al. (2017) Journal of virology 91. PMID: 29021398 3. Cartwright, JL. et al. (2002) Journal of virology 76, 1415-21. PMID: 11773415
Note	For research use only.

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SDS-PAGE for Recombinant ASFV g5Rp/D250R Protein, N-His

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Datasheet

ASFV g5Rp/D250R Recombinant Protein (N-His).pdf

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ASFV g5Rp/D250R Recombinant Protein (N-His) (VK490012)

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