Anti-ASFV g5Rp/D250R Polyclonal Antibody (VK490014)

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Overview

Catalog No.

VK490014

Description

Anti-ASFV g5Rp/D250R Polyclonal Antibody (VK490014) is a rabbit polyclonal antibody detecting D250R in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

African swine fever virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant ASFV g5Rp/D250R (Met1-His250).

Target

mRNA-decapping protein g5R, g5Rp, 3.1.3.-, ASFV-DP, Diphosphoinositol polyphosphate phosphohydrolase, DIPP, 3.6.1.52, Ba71V-102, D250R

Purification

Purified by antigen affinity column.

Accession

P32092

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

mRNA-decapping protein g5R (g5Rp/D250R) is a ~29 kDa protein. Decapping enzyme required for the removal of the 5'-end m7GpppN cap tethered to viral and host mRNAs to allow their decay in cells. May therefore accelerate viral and cellular mRNA turnover to eliminate competing host mRNAs and allow stage-specific synthesis of viral proteins. Acceleration of the turnover of cellular transcripts may even promote the shutoff of host protein synthesis. In addition to the mRNA cap, g5R also efficiently hydrolyzes diphosphoinositol polyphosphates. Down-regulation of the level of PP-InsP5 (diphosphoinositol pentakisphosphate) may play a role in viral manipulation of the cellular secretory pathway, a step necessary for the formation of virions.

1. Parrish, S. et al. (2009) Virology 393, 177-82. PMID: 19695654

2. Quintas, A. et al. (2017) Journal of virology 91. PMID: 29021398

3. Cartwright, JL. et al. (2002) Journal of virology 76, 1415-21. PMID: 11773415

Note

For research use only.

Images

Western blot

Lane 1: recombinant ASFV g5Rp/D250R (Catalog No: VK490012)

References

Datasheet

Anti-ASFV g5Rp/D250R Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ASFV g5Rp/D250R Polyclonal Antibody (VK490014)

Overview

Images

References

Datasheet

SDS

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