Anti-ATIC Polyclonal Antibody (HD440014)

Name: Anti-ATIC Polyclonal Antibody
Brand: abinScience
SKU: HD440014
Price: 96 USD
Availability: InStock

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20ug
50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HD440014

Description

Anti-ATIC Polyclonal Antibody (HD440014) is a rabbit polyclonal antibody detecting ATIC in ELISA, IHC, WB. Suitable for Rat, Mouse, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ATIC (Lys225-His592).

Cible

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase, AICAR formyltransferase, AICAR transformylase, AICAR transformylase/inosine monophosphate cyclohydrolase, ATIC, Bifunctional purine biosynthesis protein ATIC, Bifunctional purine biosynthesis protein ATIC, N-terminally processed, EC:2.1.2.3, EC:3.5.4.10, IMP cyclohydrolase, IMP synthase, Inosine 5'-monophosphate cyclohydrolase, Inosinicase, PURH, Phosphoribosylaminoimidazolecarboxamide formyltransferase

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P31939

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Noms alternatifs

P31939

Contexte

Bifunctional purine biosynthesis protein ATIC is a ~64 kDa protein. Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction. Also catalyzes the cyclization of FAICAR to inosine monophosphate (IMP). Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias.

1. Bulock, KG. et al. (2002) The Journal of biological chemistry 277, 22168-74. PMID: 11948179

2. Vergis, JM. et al. (2004) Biochemistry 43, 1184-92. PMID: 14756554

3. Wall, M. et al. (2000) Biochemistry 39, 11303-11. PMID: 10985775

4. Sugita, T. et al. (1997) Journal of biochemistry 122, 309-13. PMID: 9378707

5. Boutchueng-Djidjou, M. et al. (2015) Molecular & cellular proteomics : MCP 14, 1079-92. PMID: 25687571

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-ATIC polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HCT116 cell lysate
Lane 6: A549 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: HT-29 cell lysate
Lane 9: K562 cell lysate
Lane 10: NIH3T3 cell lysate
Lane11: PC-12 cell lysate

Reférences

Fiche technique

Anti-ATIC Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-ATIC Polyclonal Antibody (HD440014)

Aperçu

Images

Reférences

Fiche technique

SDS