Human ATIC Recombinant Protein (N-His) (HD440012)

Name: Human ATIC Recombinant Protein (N-His)
Brand: abinScience
SKU: HD440012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD440012
Description	Recombinant Human ATIC Protein, N-His (HD440012) expressed in E. coli, spanning Lys225-His592. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P31939
Protein length	Lys225-His592
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	42.64 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase, AICAR formyltransferase, AICAR transformylase, AICAR transformylase/inosine monophosphate cyclohydrolase, ATIC, Bifunctional purine biosynthesis protein ATIC, Bifunctional purine biosynthesis protein ATIC, N-terminally processed, EC:2.1.2.3, EC:3.5.4.10, IMP cyclohydrolase, IMP synthase, Inosine 5'-monophosphate cyclohydrolase, Inosinicase, PURH, Phosphoribosylaminoimidazolecarboxamide formyltransferase
Background	Bifunctional purine biosynthesis protein ATIC is a ~64 kDa protein. Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction. Also catalyzes the cyclization of FAICAR to inosine monophosphate (IMP). Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias. 1. Bulock, KG. et al. (2002) The Journal of biological chemistry 277, 22168-74. PMID: 11948179 2. Vergis, JM. et al. (2004) Biochemistry 43, 1184-92. PMID: 14756554 3. Wall, M. et al. (2000) Biochemistry 39, 11303-11. PMID: 10985775 4. Sugita, T. et al. (1997) Journal of biochemistry 122, 309-13. PMID: 9378707 5. Boutchueng-Djidjou, M. et al. (2015) Molecular & cellular proteomics : MCP 14, 1079-92. PMID: 25687571
Note	For research use only

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Human ATIC Recombinant Protein (N-His).pdf

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Human ATIC Recombinant Protein (N-His) (HD440012)

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