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| カタログ番号 | HD440014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-ATIC Polyclonal Antibody (HD440014) is a rabbit polyclonal antibody detecting ATIC in ELISA, IHC, WB. Suitable for Rat, Mouse, and Human.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ATIC (Lys225-His592). | ||||||||
| ターゲット | 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase, AICAR formyltransferase, AICAR transformylase, AICAR transformylase/inosine monophosphate cyclohydrolase, ATIC, Bifunctional purine biosynthesis protein ATIC, Bifunctional purine biosynthesis protein ATIC, N-terminally processed, EC:2.1.2.3, EC:3.5.4.10, IMP cyclohydrolase, IMP synthase, Inosine 5'-monophosphate cyclohydrolase, Inosinicase, PURH, Phosphoribosylaminoimidazolecarboxamide formyltransferase | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P31939 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 別名 | P31939 | ||||||||
| 背景 | Bifunctional purine biosynthesis protein ATIC is a ~64 kDa protein. Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction. Also catalyzes the cyclization of FAICAR to inosine monophosphate (IMP). Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias. 1. Bulock, KG. et al. (2002) The Journal of biological chemistry 277, 22168-74. PMID: 11948179 2. Vergis, JM. et al. (2004) Biochemistry 43, 1184-92. PMID: 14756554 3. Wall, M. et al. (2000) Biochemistry 39, 11303-11. PMID: 10985775 4. Sugita, T. et al. (1997) Journal of biochemistry 122, 309-13. PMID: 9378707 5. Boutchueng-Djidjou, M. et al. (2015) Molecular & cellular proteomics : MCP 14, 1079-92. PMID: 25687571 | ||||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-ATIC polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HCT116 cell lysate
Lane 6: A549 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: HT-29 cell lysate
Lane 9: K562 cell lysate
Lane 10: NIH3T3 cell lysate
Lane11: PC-12 cell lysate
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