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Anti-BCAT2 Polyclonal Antibody (HT209014)

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Vista general
Número de catálogoHT209014
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human BCAT2 (Ala107-Val392).
Objetivo BCT2, BCAT2, BCAT(m), BCATM, Placental protein 18, Branched-chain-amino-acid aminotransferase, mitochondrial, PP18, ECA40
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso O15382
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2) is a ~44 kDa protein. Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Branched chain amino acid catabolism plays a role in adipocyte differentiation by providing lipogenic acetyl-CoA pools in differentiated adipocytes. Mechanistically, acetyl-CoA derived from branched chain amino acid catabolism is used by EP300/p300 to acetylate and inhibit PRDM16, thereby preventing adipose tissue browning. May also function as a transporter of branched chain alpha-keto acids.

1. Yennawar, NH. et al. (2006) The Journal of biological chemistry 281, 39660-71. PMID: 17050531
2. Wang, XL. et al. (2015) Journal of inherited metabolic disease 38, 855-61. PMID: 25653144
3. Eden, A. et al. (1996) The Journal of biological chemistry 271, 20242-5. PMID: 8702755
Referencias 1. Lei Zhao, et al. Design, screening and biological evaluation of novel fatty acid chain-modified oxyntomodulin-based derivatives with prolonged glucose-lowering ability and potent anti-obesity effects. Org Biomol Chem. 2019, 17, 33. [HT209014]
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-BCAT2 Polyclonal Antibody

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