Human BCAT2 Recombinant Protein (N-His) (HT209012)

Name: Human BCAT2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT209012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT209012
Description	Recombinant Human BCAT2 Protein, N-His (HT209012) expressed in E. coli, spanning Ala107-Val392. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O15382
Protein length	Ala107-Val392
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.65 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	BCT2, BCAT2, BCAT(m), BCATM, Placental protein 18, Branched-chain-amino-acid aminotransferase, mitochondrial, PP18, ECA40
Background	Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2) is a ~44 kDa protein. Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Branched chain amino acid catabolism plays a role in adipocyte differentiation by providing lipogenic acetyl-CoA pools in differentiated adipocytes. Mechanistically, acetyl-CoA derived from branched chain amino acid catabolism is used by EP300/p300 to acetylate and inhibit PRDM16, thereby preventing adipose tissue browning. May also function as a transporter of branched chain alpha-keto acids. 1. Yennawar, NH. et al. (2006) The Journal of biological chemistry 281, 39660-71. PMID: 17050531 2. Wang, XL. et al. (2015) Journal of inherited metabolic disease 38, 855-61. PMID: 25653144 3. Eden, A. et al. (1996) The Journal of biological chemistry 271, 20242-5. PMID: 8702755
Note	For research use only.

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SDS-PAGE for Recombinant Human BCAT2 Protein, N-His

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Datasheet

Human BCAT2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Human BCAT2 Recombinant Protein (N-His) (HT209012)

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