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Обзор
Каталожный номерHY023014
Описание
Anti-BCR Polyclonal Antibody (HY023014) is a rabbit polyclonal antibody detecting BCR in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human BCR (Lys174-Asp331).
Цель Renal carcinoma antigen NY-REN-26, BCR1, BCR, D22S11, Breakpoint cluster region protein
Очистка Purified by antigen affinity column.
Номер доступа P11274
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Breakpoint cluster region protein (BCR) is a ~142 kDa protein. Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form.

1. Cho, YJ. et al. (2007) Molecular and cellular biology 27, 899-911. PMID: 17116687
2. Diekmann, D. et al. (1991) Nature 351, 400-2. PMID: 1903516
3. Chuang, TH. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 10282-6. PMID: 7479768
4. Dubash, AD. et al. (2013) The Journal of cell biology 202, 653-66. PMID: 23940119
5. Maru, Y. et al. (1991) Cell 67, 459-68. PMID: 1657398
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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