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Anti-BVDV N-pro Polyclonal Antibody (VK778074)

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Vista general
Número de catálogoVK778074
Descripción
Anti-BVDV N-terminal protease Polyclonal Antibody (VK778074) is a rabbit polyclonal antibody detecting BVDV N-terminal protease in WB, IHC, ELISA.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesBovine viral diarrhea virus (BVDV) (Mucosal disease virus)
AplicacionesWB,IHC,ELISA
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant BVDV N-terminal protease (Met1-Cys168).
Objetivo N-pro
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Purificación Purified by antigen affinity column.
Número de acceso P19711
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosGenome polyprotein, N-terminal protease, N-pro, EC:3.4.22.-, Autoprotease p20, Capsid protein C, p14, E(rns) glycoprotein, gp44/48, EC:4.6.1.19, Envelope glycoprotein E1, gp33, Envelope glycoprotein E2, gp55, Viroporin p7, Non-structural protein 2-3, Cysteine protease NS2, EC:3.4.22.-, Non-structural protein 2, p54, Serine protease NS3, EC:3.4.21.113, EC:3.6.1.15, EC:3.6.4.13, Non-structural protein 3, p80, Non-structural protein 4A, NS4A, p10, Non-structural protein 4B, NS4B, p30, Non-structural protein 5A, NS5A, p58, RNA-directed RNA polymerase, EC:2.7.7.48, NS5B, p75
Fondo

Genome polyprotein (N-pro) is a ~449 kDa protein. Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation.

1. Chen, Z. et al. (2007) Virology 366, 277-92. PMID: 17531282
Nota For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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