Anti-BVDV NS3/p80 Polyclonal Antibody (VK778034)

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK778034

Description

Anti-BVDV NS3/p80 Polyclonal Antibody (VK778034) is a rabbit polyclonal antibody detecting p80 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Bovine viral diarrhea virus (isolate NADL) (BVDV) (Mucosal disease virus)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant BVDV NS3/p80 (Glu1887-Thr2031).

Target

Genome polyprotein, Serine protease NS3, 3.4.21.113, 3.6.1.15, 3.6.4.13, Non-structural protein 3, p80

Purification

Purified by antigen affinity column.

Accession

P19711

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Genome polyprotein (NS3/p80) is a ~449 kDa protein. Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation.

1. Chen, Z. et al. (2007) Virology 366, 277-92. PMID: 17531282

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-BVDV-NS3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant BVDV NS3/p80 (Catalog No: VK778032)

References

Datasheet