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Anti-BVDV N-pro Polyclonal Antibody (VK778074)

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Aperçu
Numéro de catalogueVK778074
Description
Anti-BVDV N-terminal protease Polyclonal Antibody (VK778074) is a rabbit polyclonal antibody detecting BVDV N-terminal protease in WB, IHC, ELISA.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesBovine viral diarrhea virus (BVDV) (Mucosal disease virus)
ApplicationsWB,IHC,ELISA
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant BVDV N-terminal protease (Met1-Cys168).
Cible N-pro
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès P19711
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsGenome polyprotein, N-terminal protease, N-pro, EC:3.4.22.-, Autoprotease p20, Capsid protein C, p14, E(rns) glycoprotein, gp44/48, EC:4.6.1.19, Envelope glycoprotein E1, gp33, Envelope glycoprotein E2, gp55, Viroporin p7, Non-structural protein 2-3, Cysteine protease NS2, EC:3.4.22.-, Non-structural protein 2, p54, Serine protease NS3, EC:3.4.21.113, EC:3.6.1.15, EC:3.6.4.13, Non-structural protein 3, p80, Non-structural protein 4A, NS4A, p10, Non-structural protein 4B, NS4B, p30, Non-structural protein 5A, NS5A, p58, RNA-directed RNA polymerase, EC:2.7.7.48, NS5B, p75
Contexte

Genome polyprotein (N-pro) is a ~449 kDa protein. Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation.

1. Chen, Z. et al. (2007) Virology 366, 277-92. PMID: 17531282
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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