Anti-Calreticulin/CALR Polyclonal Antibody (HB190014)

Name: Anti-Calreticulin/CALR Polyclonal Antibody
Brand: abinScience
SKU: HB190014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HB190014

説明

Anti-Calreticulin/CALR Polyclonal Antibody (HB190014) is a rabbit polyclonal antibody detecting Calreticulin in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human Calreticulin/CALR (Glu18-Pro204).

ターゲット

Calreticulin, ERp60, CRTC, Calregulin, grp60, HACBP, Endoplasmic reticulum resident protein 60, CALR, CRP55

精製

Purified by antigen affinity column.

アクセッション番号

P27797

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Calreticulin is a ~48 kDa protein. Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.

1. Nauseef, WM. et al. (1995) The Journal of biological chemistry 270, 4741-7. PMID: 7876246

2. Holaska, JM. et al. (2001) The Journal of cell biology 152, 127-40. PMID: 11149926

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-CALR polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lane 3: HL-60 cell lysate

参考文献

データシート

Anti-Calreticulin/CALR Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Calreticulin/CALR Polyclonal Antibody (HB190014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ