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Anti-Calreticulin/CALR Polyclonal Antibody
Anti-Calreticulin/CALR Polyclonal Antibody
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Спецификация:
  • 20ug
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHB190014
Описание
Anti-Calreticulin/CALR Polyclonal Antibody (HB190014) is a rabbit polyclonal antibody detecting Calreticulin in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cricetulus griseus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human Calreticulin/CALR (Glu18-Pro204).
Цель Calreticulin, ERp60, CRTC, Calregulin, grp60, HACBP, Endoplasmic reticulum resident protein 60, CALR, CRP55
Очистка Purified by antigen affinity column.
Номер доступа P27797
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Calreticulin is a ~48 kDa protein. Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.

1. Nauseef, WM. et al. (1995) The Journal of biological chemistry 270, 4741-7. PMID: 7876246
2. Holaska, JM. et al. (2001) The Journal of cell biology 152, 127-40. PMID: 11149926
Примечание For research use only.
Изображения
  • Anti-Calreticulin/CALR Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CALR polyclonal antibody at 1ug/mL on various samples.
    Lane 1: SH-SY5Y cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HL-60 cell lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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