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Anti-CD10/MME Polyclonal Antibody (HY401014)

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Vista general
Número de catálogoHY401014
Descripción
Anti-CD10/MME Polyclonal Antibody (HY401014) is a rabbit polyclonal antibody detecting CD10 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Pongo abelii, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CD10/MME (Met1-Cys80).
Objetivo Atriopeptidase, Common acute lymphocytic leukemia antigen, Neutral endopeptidase, Skin fibroblast elastase, CD10, SFE, CALLA, Neprilysin, Neutral endopeptidase 24.11, MME, NEP, Enkephalinase, EPN
Purificación Purified by antigen affinity column.
Número de acceso P08473
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Neprilysin (CD10/MME) is a ~85 kDa protein. Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)).

1. Rice, GI. et al. (2004) The Biochemical journal 383, 45-51. PMID: 15283675
2. Skidgel, RA. et al. (1984) Peptides 5, 769-76. PMID: 6208535
3. Gafford, JT. et al. (1983) Biochemistry 22, 3265-71. PMID: 6349683
4. Le Moual, H. et al. (1994) European journal of biochemistry 221, 475-80. PMID: 8168535
5. Wisner, A. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17979-84. PMID: 17101991
6. Brandt, I. et al. (2006) Clinical chemistry 52, 82-7. PMID: 16254193
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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