Anti-Human Neprilysin/CD10 Recombinant Antibody (NL-1) (HY401107)

Name: Anti-Human Neprilysin/CD10 Recombinant Antibody (NL-1)
Brand: abinScience
SKU: HY401107
Price: 196 USD
Availability: InStock

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Overview

Catalog No.	HY401107
Description	Anti-Human Neprilysin/CD10 Recombinant Antibody (NL-1) (HY401107) is a mouse monoclonal antibody detecting Neprilysin/CD10 in FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone ID	NL-1
Target	Atriopeptidase, Common acute lymphocytic leukemia antigen, Neutral endopeptidase, Skin fibroblast elastase, CD10, SFE, CALLA, Neprilysin, Neutral endopeptidase 24.11, MME, NEP, Enkephalinase, EPN
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P08473
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Neprilysin (CD10/MME) is a ~85 kDa protein. Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). 1. Rice, GI. et al. (2004) The Biochemical journal 383, 45-51. PMID: 15283675 2. Skidgel, RA. et al. (1984) Peptides 5, 769-76. PMID: 6208535 3. Gafford, JT. et al. (1983) Biochemistry 22, 3265-71. PMID: 6349683 4. Le Moual, H. et al. (1994) European journal of biochemistry 221, 475-80. PMID: 8168535 5. Wisner, A. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17979-84. PMID: 17101991 6. Brandt, I. et al. (2006) Clinical chemistry 52, 82-7. PMID: 16254193
Note	For research use only.

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SEC-HPLC

SEC-HPLC detection for Anti-Human CD10/MME Antibody (NL-1).
Bioactivity

Detects CD10/MME in indirect ELISAs.
Flow CytoMetry

Flow-cytometry using anti-human CD10 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD10 monoclonal antibody (Catalog: HY401107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human Neprilysin/CD10 Recombinant Antibody (NL-1).pdf

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Recombinant Proteins

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Anti-Human Neprilysin/CD10 Recombinant Antibody (NL-1) (HY401107)

Overview

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References

Datasheet

SDS