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Anti-Human MDR1/ABCB1 Polyclonal Antibody (HY466014)

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Vista general
Número de catálogoHY466014
Descripción
Anti-Human MDR1/ABCB1 Polyclonal Antibody (HY466014) is a rabbit polyclonal antibody detecting MDR1/ABCB1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CD243/ABCB1 (Phe394-Val672).
Objetivo Phospholipid transporter ABCB1, MDR1, CD243, ATP-binding cassette sub-family B member 1, ABCB1, Multidrug resistance protein 1, P-glycoprotein 1, ATP-dependent translocase ABCB1, PGY1
Purificación Purified by antigen affinity column.
Número de acceso P08183
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

ATP-dependent translocase ABCB1 (CD243) is a ~141 kDa protein. Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

1. Choi, KH. et al. (1988) Cell 53, 519-29. PMID: 2897240
2. Masud, SN. et al. (2022) Nature chemical biology 18, 1370-1379. PMID: 35970996
3. van Helvoort, A. et al. (1996) Cell 87, 507-17. PMID: 8898203
4. Chen, G. et al. (1997) The Journal of biological chemistry 272, 5974-82. PMID: 9038218
5. Shafik, SH. et al. (2022) PLoS biology 20, e3001616. PMID: 35507548
Nota For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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