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Anti-CHD4 Polyclonal Antibody (HC562014)

Anti-CHD4 Polyclonal Antibody
Anti-CHD4 Polyclonal Antibody
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Especificación:
  • 20ug
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoHC562014
Descripción
Anti-CHD4 Polyclonal Antibody (HC562014) is a rabbit polyclonal antibody detecting CHD4 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CHD4 (Thr1706-Gln1912).
Objetivo ATP-dependent helicase CHD4, CHD-4, CHD4, Chromodomain-helicase-DNA-binding protein 4, EC:3.6.4.-, Mi-2 autoantigen 218 kDa protein, Mi2-beta
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q14839
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosQ14839
Fondo

ATP-dependent chromatin remodeler CHD4 is a ~218 kDa protein. ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination. Involved in neurogenesis.

1. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666
2. Farnung, L. et al. (2020) eLife 9. PMID: 32543371
3. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440
4. Sillibourne, JE. et al. (2007) Molecular biology of the cell 18, 3667-80. PMID: 17626165
5. Tong, JK. et al. (1998) Nature 395, 917-21. PMID: 9804427
6. Gong, F. et al. (2015) Genes & development 29, 197-211. PMID: 25593309
Nota For research use only
Imágenes
  • Anti-CHD4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CHD4 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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