Anti-CKMT1A Polyclonal Antibody (HY409014)

Name: Anti-CKMT1A Polyclonal Antibody
Brand: abinScience
SKU: HY409014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HY409014

Описание

Anti-CKMT1A Polyclonal Antibody (HY409014) is a rabbit polyclonal antibody detecting CKMT1A in ELISA, IHC, WB. Suitable for Bovine, Chicken, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human CKMT1A (Ser41-Ile250).

Цель

U-MtCK, CKMT1A, CKMT, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, Mia-CK, Creatine kinase U-type, mitochondrial

Очистка

Purified by antigen affinity column.

Номер доступа

P12532

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Creatine kinase U-type, mitochondrial (CKMT1A) is a ~47 kDa protein. Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Примечание

For research use only.

Изображения

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CKMT1A (HY409014) at a concentration of 12.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CKMT1A was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

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