Anti-Clostridioides difficile Toxin B/tcdB Recombinant Nanobody (SAA1173) (JN876013)

Name: Anti-Clostridioides difficile Toxin B/tcdB Recombinant Nanobody (SAA1173)
Brand: abinScience
SKU: JN876013
Price: 126 USD
Availability: InStock

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50ug
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1mg

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SDS

Обзор

Каталожный номер

JN876013

Описание

Anti-Clostridioides difficile Toxin B/tcdB Nanobody (SAA1173) [SAA1173] (JN876013) is a alpaca monoclonal antibody detecting Clostridium difficile Toxin in ELISA, SPR, WB.

Highlights

●Multi-Application — Validated across multiple applications.

Реактивность видов

Clostridioides difficile

Применения

ELISA, SPR, WB

Хозяинский вид

Alpaca

Клоональность

Monoclonal

Изотип

VHH-8His-Cys-tag

ID клона

SAA1173

Цель

Toxin B, 3.4.22.-, Glucosyltransferase TcdB, 2.4.1.-, tcdB, toxB, Clostridium difficile Toxin

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Purified by Nickel column.

Номер доступа

P18177

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149

3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933

5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463

6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

Примечание

For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Anti-Clostridioides difficile Toxin B/tcdB Nanobody (SAA1173)

Ссылки

Даташит

Anti-Clostridioides difficile Toxin B/tcdB Recombinant Nanobody (SAA1173).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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