Anti-Clostridioides difficile Toxin B/tcdB Polyclonal Antibody (JN876014)

Name: Anti-Clostridioides difficile Toxin B/tcdB Polyclonal Antibody
Brand: abinScience
SKU: JN876014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

JN876014

Species reactivity

Clostridioides difficile (Peptoclostridium difficile)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Clostridioides difficile Toxin B/tcdB (Gly1834-Glu2053).

Target

Toxin B, 3.4.22.-, Glucosyltransferase TcdB, 2.4.1.-, tcdB, toxB, Clostridium difficile Toxin

Purification

Purified by antigen affinity column.

Accession

P18177

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149

3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933

5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463

6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Clostridioides difficile Toxin B/tcdB polyclonal antibody at 1ug/mL on recombinant Clostridioides difficile Toxin B/tcdB (Catalog No. JN876022).
Western blot

Western blot analysis was performed using anti-Toxin B polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridioides difficile Toxin B/tcdB (Catalog No: JN876022)