Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0938) (JN959023)

Name: Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0938)
Brand: abinScience
SKU: JN959023
Price: 191 USD
Availability: InStock

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100ug
1mg

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN959023

Description

Anti-Clostridium botulinum BoNT/B Nanobody (SAA0938) [SAA0938] (JN959023) is a alpaca monoclonal antibody detecting BoNT/B in ELISA.

Reactivité des espèces

Clostridium botulinum

Applications

ELISA

Espèce hôte

Alpaca

Clonalité

Monoclonal

Isotype

VHH-8His-Cys-tag

ID de clone

SAA0938

Cible

Botulinum neurotoxin type B, BoNT/B, Bontoxilysin-B, Botulinum neurotoxin B light chain, LC, 3.4.24.69, Botulinum neurotoxin B heavy chain, HC, botB

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Numéro d'accès

P10844

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Botulinum neurotoxin type B (BoNT) is a ~150 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin B which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Chai, Q. et al. (2006) Nature 444, 1096-100. PMID: 17167418

2. Jin, R. et al. (2006) Nature 444, 1092-5. PMID: 17167421

3. Rummel, A. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 359-64. PMID: 17185412

4. Berntsson, RP. et al. (2013) Nature communications 4, 2058. PMID: 23807078

5. Dong, M. et al. (2003) The Journal of cell biology 162, 1293-303. PMID: 14504267

6. Hoch, DH. et al. (1985) Proceedings of the National Academy of Sciences of the United States of America 82, 1692-6. PMID: 3856850

7. Nishiki, T. et al. (1994) The Journal of biological chemistry 269, 10498-503. PMID: 8144634

10. Sun, S. et al. (2012) Biochemistry 51, 5655-62. PMID: 22720883

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Clostridium botulinum BoNT/B Nanobody (SAA0938)

Reférences

Fiche technique

Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0938).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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