Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941) (JN959053)

Name: Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941)
Brand: abinScience
SKU: JN959053
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

JN959053

Description

Anti-Clostridium botulinum BoNT/B Nanobody (SAA0941) [SAA0941] (JN959053) is a alpaca monoclonal antibody detecting BoNT/B in ELISA.

Species reactivity

Clostridium botulinum

Applications

ELISA

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA0941

Clonality

Monoclonal

Target

Botulinum neurotoxin type B, BoNT/B, Bontoxilysin-B, Botulinum neurotoxin B light chain, LC, 3.4.24.69, Botulinum neurotoxin B heavy chain, HC, botB

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P10844

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Botulinum neurotoxin type B (BoNT) is a ~150 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin B which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Chai, Q. et al. (2006) Nature 444, 1096-100. PMID: 17167418

2. Jin, R. et al. (2006) Nature 444, 1092-5. PMID: 17167421

3. Rummel, A. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 359-64. PMID: 17185412

4. Berntsson, RP. et al. (2013) Nature communications 4, 2058. PMID: 23807078

5. Dong, M. et al. (2003) The Journal of cell biology 162, 1293-303. PMID: 14504267

6. Hoch, DH. et al. (1985) Proceedings of the National Academy of Sciences of the United States of America 82, 1692-6. PMID: 3856850

7. Nishiki, T. et al. (1994) The Journal of biological chemistry 269, 10498-503. PMID: 8144634

10. Sun, S. et al. (2012) Biochemistry 51, 5655-62. PMID: 22720883

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Clostridium botulinum BoNT/B Nanobody (SAA0941)

References

Datasheet

Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941) (JN959053)

Overview

Images

References

Datasheet

SDS

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