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| カタログ番号 | JN959033 | ||||
|---|---|---|---|---|---|
| 説明 |
Anti-Clostridium botulinum BoNT/B Nanobody (SAA0939) [SAA0939] (JN959033) is a alpaca monoclonal antibody detecting BoNT/B in ELISA.
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| 種反応性 | Clostridium botulinum | ||||
| アプリケーション | ELISA | ||||
| 宿主種 | Alpaca | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | VHH-8His-Cys-tag | ||||
| クローンID | SAA0939 | ||||
| ターゲット | Botulinum neurotoxin type B, BoNT/B, Bontoxilysin-B, Botulinum neurotoxin B light chain, LC, 3.4.24.69, Botulinum neurotoxin B heavy chain, HC, botB | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Purified by Nickel column. | ||||
| アクセッション番号 | P10844 | ||||
| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 背景 | Botulinum neurotoxin type B (BoNT) is a ~150 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin B which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. 1. Chai, Q. et al. (2006) Nature 444, 1096-100. PMID: 17167418 2. Jin, R. et al. (2006) Nature 444, 1092-5. PMID: 17167421 3. Rummel, A. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 359-64. PMID: 17185412 4. Berntsson, RP. et al. (2013) Nature communications 4, 2058. PMID: 23807078 5. Dong, M. et al. (2003) The Journal of cell biology 162, 1293-303. PMID: 14504267 6. Hoch, DH. et al. (1985) Proceedings of the National Academy of Sciences of the United States of America 82, 1692-6. PMID: 3856850 7. Nishiki, T. et al. (1994) The Journal of biological chemistry 269, 10498-503. PMID: 8144634 10. Sun, S. et al. (2012) Biochemistry 51, 5655-62. PMID: 22720883 | ||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Clostridium botulinum BoNT/B Nanobody (SAA0939)
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