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| カタログ番号 | JN963023 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | Clostridium perfringens | ||||||
| アプリケーション | ELISA, Neutralization, WB | ||||||
| 宿主種 | Mouse | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG2a | ||||||
| クローンID | HS3 | ||||||
| ターゲット | Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163, Clostridium perfringens (strain 13 / Type A) | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | P0C2E9 | ||||||
| RRID | Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3) (abinScience Cat# JN963023, RRID:AB_3729018) |
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| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 背景 | Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation. | ||||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3)
Detects recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012) in indirect ELISA
Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
Lane 1: ATG5659 200ng
Lane 2: 1* loading buffer
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