Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Polyclonal Antibody (JN963014)

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50ug
100ug

Number:

Overview
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Datasheet
SDS

Overview

Catalog No.

JN963014

Description

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Polyclonal Antibody (JN963014) is a rabbit polyclonal antibody detecting Theta-toxin in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Clostridium perfringens

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Asp30-Asn500).

Target

Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163

Purification

Purified by antigen affinity column.

Accession

P0C2E9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin polyclonal antibody at 1ug/mL on recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No. JN963012).
Western blot

Western blot analysis was performed using anti-PFO polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012)