Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS3) (JN963023)

Name: Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS3)
Brand: abinScience
SKU: JN963023
Price: 126 USD
Availability: InStock

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Спецификация:

20ug
50ug
100ug
1mg

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

JN963023

Реактивность видов

Clostridium perfringens

Применения

ELISA, Neutralization, WB

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG2a

ID клона

HS3

Цель

Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163, Clostridium perfringens (strain 13 / Type A)

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P0C2E9

RRID

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3) (abinScience Cat# JN963023, RRID:AB_3729018)
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Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

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Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Примечание

For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3)
Bioactivity

Detects recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012) in indirect ELISA
Western blot

Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
Lane 1: ATG5659 200ng
Lane 2: 1* loading buffer