Anti-CTNNB1 Polyclonal Antibody (HW518014)

Name: Anti-CTNNB1 Polyclonal Antibody
Brand: abinScience
SKU: HW518014
Price: 96 USD
Availability: InStock

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HW518014

Description

Anti-CTNNB1 Polyclonal Antibody (HW518014) is a rabbit polyclonal antibody detecting CTNNB1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human CTNNB1 (Met1-Leu781).

Cible

CTNNB, Catenin beta-1, CTNNB1, Beta-catenin

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P35222, Q02248, Q9WU82

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Catenin beta-1 (CTNNB1) is a ~85 kDa protein. Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Also acts as a coactivator for other transcription factors, such as NR5A2. Promotes epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via driving transcription of CTNNB1/TCF-target genes.

1. Lillehoj, EP. et al. (2007) Biochimica et biophysica acta 1773, 1028-38. PMID: 17524503

2. Weiske, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 20344-9. PMID: 18077326

3. Bahmanyar, S. et al. (2008) Genes & development 22, 91-105. PMID: 18086858

4. Li, H. et al. (2009) The Journal of biological chemistry 284, 2012-22. PMID: 18957423

5. Fiset, A. et al. (2011) Cellular signalling 23, 911-9. PMID: 21262353

6. Satow, R. et al. (2012) Gastroenterology 142, 572-81. PMID: 22155184

7. Genovese, G. et al. (2012) Cell cycle (Georgetown, Tex.) 11, 2206-15. PMID: 22647378

8. Yu, Y. et al. (2012) EMBO reports 13, 750-8. PMID: 22699938

10. Park, J. et al. (2018) Stem cell reports 11, 115-127. PMID: 29910125

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CTNNB1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: Lncap cell lysate

Reférences

Fiche technique

Anti-CTNNB1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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