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Anti-CYP21A2 Polyclonal Antibody (HY097014)

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Vista general
Número de catálogoHY097014
Descripción
Anti-CYP21A2 Polyclonal Antibody (HY097014) is a rabbit polyclonal antibody detecting CYP21A2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CYP21A2 (Leu107-Gly390).
Objetivo CYP21, CYP21B, Cytochrome P450 21, Cytochrome P450-C21B, Cytochrome P450-C21, Cytochrome P450 XXI, 21-OHase, Steroid 21-hydroxylase, Cytochrome P-450c21, CYP21A2
Purificación Purified by antigen affinity column.
Número de acceso P08686
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Steroid 21-hydroxylase (CYP21A2) is a ~56 kDa protein. A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

1. Tajima, T. et al. (1999) Gene therapy 6, 1898-903. PMID: 10602386
2. Grischuk, Y. et al. (2006) The Journal of clinical endocrinology and metabolism 91, 4976-80. PMID: 16984992
3. Concolino, P. et al. (2012) Metabolism: clinical and experimental 61, 519-24. PMID: 22014889
4. Pallan, PS. et al. (2015) The Journal of biological chemistry 290, 13128-43. PMID: 25855791
5. de Paula Michelatto, D. et al. (2016) International journal of endocrinology 2016, 4209670. PMID: 27721825
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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