Human CYP21A2 Recombinant Protein (N-His) (HY097012)

Name: Human CYP21A2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY097012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY097012
Description	Recombinant Human CYP21A2 Protein, N-His (HY097012) expressed in E. coli, spanning Leu107-Gly390. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P08686
Protein length	Leu107-Gly390
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.46 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CYP21, CYP21B, Cytochrome P450 21, Cytochrome P450-C21B, Cytochrome P450-C21, Cytochrome P450 XXI, 21-OHase, Steroid 21-hydroxylase, Cytochrome P-450c21, CYP21A2
Background	Steroid 21-hydroxylase (CYP21A2) is a ~56 kDa protein. A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). 1. Tajima, T. et al. (1999) Gene therapy 6, 1898-903. PMID: 10602386 2. Grischuk, Y. et al. (2006) The Journal of clinical endocrinology and metabolism 91, 4976-80. PMID: 16984992 3. Concolino, P. et al. (2012) Metabolism: clinical and experimental 61, 519-24. PMID: 22014889 4. Pallan, PS. et al. (2015) The Journal of biological chemistry 290, 13128-43. PMID: 25855791 5. de Paula Michelatto, D. et al. (2016) International journal of endocrinology 2016, 4209670. PMID: 27721825
Note	For research use only.

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SDS-PAGE for Recombinant Human CYP21A2 Protein, N-His

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Datasheet

Human CYP21A2 Recombinant Protein (N-His).pdf

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Human CYP21A2 Recombinant Protein (N-His) (HY097012)

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