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Anti-Human DGKZ Polyclonal Antibody (HC693014)

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Vista general
Número de catálogoHC693014
Descripción
Anti-DGKZ Polyclonal Antibody (HC693014) is a rabbit polyclonal antibody detecting DGKZ in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human DGKZ (Gly411-Lys624).
Objetivo DAGK6, DAG kinase zeta, Diglyceride kinase zeta, DGK-zeta, Diacylglycerol kinase zeta, DGKZ
Purificación Purified by antigen affinity column.
Número de acceso Q13574
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Diacylglycerol kinase zeta (DGKZ) is a ~103 kDa protein. Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group.

1. Epand, RM. et al. (2004) Biochemistry 43, 14778-83. PMID: 15544348
2. Epand, RM. et al. (2007) Biochemistry 46, 14225-31. PMID: 18004883
3. Lung, M. et al. (2009) The Journal of biological chemistry 284, 31062-73. PMID: 19744926
4. Shulga, YV. et al. (2011) FEBS letters 585, 4025-8. PMID: 22108654
5. Gellett, AM. et al. (2012) Biochemical and biophysical research communications 422, 758-63. PMID: 22627129
6. Sato, M. et al. (2013) Pharmacology 92, 99-107. PMID: 23949095
7. Ding, L. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 5519-24. PMID: 9159104
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human DGKZ Polyclonal Antibody

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