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Anti-Human DGKZ Polyclonal Antibody (HC693024)

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Overview
Catalog No.HC693024
Description
Anti-Human DGKZ Polyclonal Antibody (HC693024) is a rabbit polyclonal antibody detecting DGKZ. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human DGKZ (Gly411-Lys624).
Target DAG kinase zeta, DAGK6, DGK-zeta, DGKZ, Diacylglycerol kinase zeta, Diglyceride kinase zeta, EC:2.7.1.107, EC:2.7.1.93
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q13574
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Diacylglycerol kinase zeta (DGKZ) is a ~103 kDa protein. Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group.

1. Epand, RM. et al. (2004) Biochemistry 43, 14778-83. PMID: 15544348
2. Epand, RM. et al. (2007) Biochemistry 46, 14225-31. PMID: 18004883
3. Lung, M. et al. (2009) The Journal of biological chemistry 284, 31062-73. PMID: 19744926
4. Shulga, YV. et al. (2011) FEBS letters 585, 4025-8. PMID: 22108654
5. Gellett, AM. et al. (2012) Biochemical and biophysical research communications 422, 758-63. PMID: 22627129
6. Sato, M. et al. (2013) Pharmacology 92, 99-107. PMID: 23949095
7. Ding, L. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 5519-24. PMID: 9159104
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human DGKZ Polyclonal Antibody

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