Anti-DNA Recombinant Antibody (3E10#) (YP539203)

Name: Anti-DNA Recombinant Antibody (3E10#)
Brand: abinScience
SKU: YP539203
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

YP539203

Aplicaciones

DB, ELISA, IF

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a, kappa

ID de clon

3E10#

Objetivo

DNA, dsDNA

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

RRID

Anti-dsDNA Antibody (3E10#) (abinScience Cat# YP539203, RRID:AB_3725561)

Forma

Liquid

Buffer de almacenamiento

0.94 mM Citric acid monohydrate, 16.7mM Trisodium citrate dihydrate, 6.2% sucrose, pH 6.5, 0.01% Tween 80

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-dsDNA Antibody (3E10#).
Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Immunofluorescence

MG63 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).