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Каталожный номерHX082014
Описание
Anti-E2F1/RBBP3 Polyclonal Antibody (HX082014) is a rabbit polyclonal antibody detecting E2F1/RBBP3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human E2F1/RBBP3 (Val88-Phe437).
Цель Retinoblastoma-binding protein 3, Retinoblastoma-associated protein 1, RBBP-3, Transcription factor E2F1, RBBP3, E2F1, E2F-1, RBAP-1, pRB-binding protein E2F-1, PBR3
Очистка Purified by antigen affinity column.
Номер доступа Q01094
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Transcription factor E2F1 (RBBP3) is a ~46 kDa protein. Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters.

1. Martínez-Balbás, MA. et al. (2000) The EMBO journal 19, 662-71. PMID: 10675335
2. Stevens, C. et al. (2003) Nature cell biology 5, 401-9. PMID: 12717439
3. García-Alvarez, G. et al. (2007) Biochimica et biophysica acta 1773, 375-82. PMID: 17050006
4. Wang, C. et al. (2007) The Journal of biological chemistry 282, 29902-9. PMID: 17704056
5. Wang, C. et al. (2008) FEBS letters 582, 2793-8. PMID: 18625225
6. Wang, D. et al. (2018) Journal of molecular cell biology 10, 60-73. PMID: 28992046
8. Zaragoza, K. et al. (2010) Molecular and cellular biology 30, 2293-304. PMID: 20176812
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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