Human E2F1 Recombinant Protein (N-His) (HX082012)

Name: Human E2F1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX082012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX082012
Description	Recombinant Human E2F1 Protein, N-His (HX082012) expressed in E. coli, spanning Val264-Phe437. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q01094
Protein length	Val264-Phe437
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	21.11 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Retinoblastoma-binding protein 3, Retinoblastoma-associated protein 1, RBBP-3, Transcription factor E2F1, RBBP3, E2F1, E2F-1, RBAP-1, pRB-binding protein E2F-1, PBR3
Background	Transcription factor E2F1 (RBBP3) is a ~46 kDa protein. Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. 1. Martínez-Balbás, MA. et al. (2000) The EMBO journal 19, 662-71. PMID: 10675335 2. Stevens, C. et al. (2003) Nature cell biology 5, 401-9. PMID: 12717439 3. García-Alvarez, G. et al. (2007) Biochimica et biophysica acta 1773, 375-82. PMID: 17050006 4. Wang, C. et al. (2007) The Journal of biological chemistry 282, 29902-9. PMID: 17704056 5. Wang, C. et al. (2008) FEBS letters 582, 2793-8. PMID: 18625225 6. Wang, D. et al. (2018) Journal of molecular cell biology 10, 60-73. PMID: 28992046 8. Zaragoza, K. et al. (2010) Molecular and cellular biology 30, 2293-304. PMID: 20176812
Note	For research use only.