Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (VK536014)

Name: Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody
Brand: abinScience
SKU: VK536014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

VK536014

Descripción

Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (VK536014) is a rabbit polyclonal antibody detecting BZLF1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Epstein-Barr virus (HHV-4) (Human herpesvirus 4)

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant EBV/HHV-4 BZLF1 (Leu175-Phe245).

Objetivo

Lytic switch protein BZLF1, EB1, Protein Z, Trans-activator protein BZLF1, Zta, bZIP transcription factor ZEBRA, BZLF1

Purificación

Purified by antigen affinity column.

Número de acceso

P03206

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Lytic switch protein BZLF1 is a ~26 kDa protein. Transcription factor that acts as a molecular switch to induce the transition from the latent to the lytic or productive phase of the virus cycle (Probable). Mediates the switch from the latent to the lytic cycle of infection in cells containing a highly methylated viral genome. Probably binds to silenced chromatin and recruits host chromatin-remodeling enzymes. Regulates this switch by binding to 2 types of ZEBRA response elements (ZREs): the CpG-free AP-1 like elements (latency) and the methylated CpG-containing elements (lytic replication) (Probable). Activates preferentially the methylated forms of the viral lytic R (BRLF1) and Na (BRRF1) gene promoters, the latters being the first genes activated during Z-mediated reactivation in latently infected cells.

1. Schepers, A. et al. (1993) The EMBO journal 12, 3921-9. PMID: 8404860

2. Bhende, PM. et al. (2004) Nature genetics 36, 1099-104. PMID: 15361873

3. Bernaudat, F. et al. (2022) Nucleic acids research 50, 490-511. PMID: 34893887

4. Schaeffner, M. et al. (2019) Life science alliance 2. PMID: 30926617

5. Flower, K. et al. (2011) PloS one 6, e25922. PMID: 22022468

6. Dickerson, SJ. et al. (2009) PLoS pathogens 5, e1000356. PMID: 19325883

7. Feederle, R. et al. (2000) The EMBO journal 19, 3080-9. PMID: 10856251

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-BLZF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EBV/HHV-4 BZLF1 (Catalog No: VK536012)

Referencias

Datasheet

Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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