EBV/HHV-4 BZLF1 Recombinant Protein (N-GST & C-His) (VK536012)

Name: EBV/HHV-4 BZLF1 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: VK536012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK536012
Description	Recombinant EBV/HHV-4 BZLF1 Protein, N-GST & C-His (VK536012) expressed in E. coli, spanning Leu175-Phe245. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P03206
Protein length	Leu175-Phe245
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.56 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Lytic switch protein BZLF1, EB1, Protein Z, Trans-activator protein BZLF1, Zta, bZIP transcription factor ZEBRA, BZLF1
Background	Lytic switch protein BZLF1 is a ~26 kDa protein. Transcription factor that acts as a molecular switch to induce the transition from the latent to the lytic or productive phase of the virus cycle (Probable). Mediates the switch from the latent to the lytic cycle of infection in cells containing a highly methylated viral genome. Probably binds to silenced chromatin and recruits host chromatin-remodeling enzymes. Regulates this switch by binding to 2 types of ZEBRA response elements (ZREs): the CpG-free AP-1 like elements (latency) and the methylated CpG-containing elements (lytic replication) (Probable). Activates preferentially the methylated forms of the viral lytic R (BRLF1) and Na (BRRF1) gene promoters, the latters being the first genes activated during Z-mediated reactivation in latently infected cells. 1. Schepers, A. et al. (1993) The EMBO journal 12, 3921-9. PMID: 8404860 2. Bhende, PM. et al. (2004) Nature genetics 36, 1099-104. PMID: 15361873 3. Bernaudat, F. et al. (2022) Nucleic acids research 50, 490-511. PMID: 34893887 4. Schaeffner, M. et al. (2019) Life science alliance 2. PMID: 30926617 5. Flower, K. et al. (2011) PloS one 6, e25922. PMID: 22022468 6. Dickerson, SJ. et al. (2009) PLoS pathogens 5, e1000356. PMID: 19325883 7. Feederle, R. et al. (2000) The EMBO journal 19, 3080-9. PMID: 10856251
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant EBV/HHV-4 BZLF1 Protein, N-GST & C-His

References

VK536012-SDS-PAGE-1.jpg

Datasheet

EBV/HHV-4 BZLF1 Recombinant Protein (N-GST & C-His).pdf

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Formula

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EBV/HHV-4 BZLF1 Recombinant Protein (N-GST & C-His) (VK536012)

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