Anti-ENO1 Polyclonal Antibody (HY081014)

Name: Anti-ENO1 Polyclonal Antibody
Brand: abinScience
SKU: HY081014
Price: 96 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HY081014

Description

Anti-ENO1 Polyclonal Antibody (HY081014) is a rabbit polyclonal antibody detecting ENO1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Anas platyrhynchos, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ENO1 (Pro267-Leu354).

Cible

Non-neural enolase, 2-phospho-D-glycerate hydro-lyase, MPB-1, ENO1, Plasminogen-binding protein, Enolase 1, C-myc promoter-binding protein, Alpha-enolase, MBP-1, MBPB1, MPB1, ENO1L1, NNE, Phosphopyruvate hydratase

Purification

Purified by antigen affinity column.

Numéro d'accès

P06733

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Alpha-enolase (ENO1) is a ~47 kDa protein. Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Also involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

1. Sugahara, T. et al. (1992) Cytotechnology 10, 137-46. PMID: 1369209

2. Huang, H. et al. (2018) Molecular cell 70, 663-678.e6. PMID: 29775581

3. Feo, S. et al. (2000) FEBS letters 473, 47-52. PMID: 10802057

4. López-Alemany, R. et al. (2003) American journal of hematology 72, 234-42. PMID: 12666133

5. Ray, R. et al. (1991) Molecular and cellular biology 11, 2154-61. PMID: 2005901

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ENO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: LO2 cell lysate
Lane 3: PC-3 cell lysate
Lane 4: SGC-7901 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: Mouse lung lysate
Lane 7: Mouse skeletal muscle lysate
Lane 8: Mouse brain lysate
Lane 9: Rat lung lysate
Lane 10: Rat skeletal muscle lysate
Lane11: Rat brain lysate