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Recombinant Human ENO1/Alpha-enolase Protein, C-MAT (HY081011)

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Recombinant Human ENO1/Alpha-enolase Protein, C-MAT

Recombinant Human ENO1/Alpha-enolase Protein, C-MAT

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Datasheet

Overview

Catalog No.	HY081011
Description	Recombinant Human ENO1/Alpha-enolase Protein, C-MAT (HY081011) expressed in Insect Cells, spanning Met1-Lys434. Purity: >90% by SDS-PAGE. Highlights ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Insect Cells
Accession	P06733
Protein length	Met1-Lys434
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	48.39 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Non-neural enolase, 2-phospho-D-glycerate hydro-lyase, MPB-1, ENO1, Plasminogen-binding protein, Enolase 1, C-myc promoter-binding protein, Alpha-enolase, MBP-1, MBPB1, MPB1, ENO1L1, NNE, Phosphopyruvate hydratase
Background	Alpha-enolase (ENO1) is a ~47 kDa protein. Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Also involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. 1. Sugahara, T. et al. (1992) Cytotechnology 10, 137-46. PMID: 1369209 2. Huang, H. et al. (2018) Molecular cell 70, 663-678.e6. PMID: 29775581 3. Feo, S. et al. (2000) FEBS letters 473, 47-52. PMID: 10802057 4. López-Alemany, R. et al. (2003) American journal of hematology 72, 234-42. PMID: 12666133 5. Ray, R. et al. (1991) Molecular and cellular biology 11, 2154-61. PMID: 2005901
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human ENO1/Alpha-enolase Protein, C-MAT

References

HY081011-SDS-PAGE-1.jpg

Datasheet

Recombinant Human ENO1/Alpha-enolase Protein, C-MAT.pdf

Formula

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Recombinant Human ENO1/Alpha-enolase Protein, C-MAT