Anti-ENO1 Polyclonal Antibody (HY081014)

Name: Anti-ENO1 Polyclonal Antibody
Brand: abinScience
SKU: HY081014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY081014

Description

Anti-ENO1 Polyclonal Antibody (HY081014) is a rabbit polyclonal antibody detecting ENO1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Anas platyrhynchos, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ENO1 (Pro267-Leu354).

Target

Non-neural enolase, 2-phospho-D-glycerate hydro-lyase, MPB-1, ENO1, Plasminogen-binding protein, Enolase 1, C-myc promoter-binding protein, Alpha-enolase, MBP-1, MBPB1, MPB1, ENO1L1, NNE, Phosphopyruvate hydratase

Purification

Purified by antigen affinity column.

Accession

P06733

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Alpha-enolase (ENO1) is a ~47 kDa protein. Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Also involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

1. Sugahara, T. et al. (1992) Cytotechnology 10, 137-46. PMID: 1369209

2. Huang, H. et al. (2018) Molecular cell 70, 663-678.e6. PMID: 29775581

3. Feo, S. et al. (2000) FEBS letters 473, 47-52. PMID: 10802057

4. López-Alemany, R. et al. (2003) American journal of hematology 72, 234-42. PMID: 12666133

5. Ray, R. et al. (1991) Molecular and cellular biology 11, 2154-61. PMID: 2005901

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ENO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: LO2 cell lysate
Lane 3: PC-3 cell lysate
Lane 4: SGC-7901 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: Mouse lung lysate
Lane 7: Mouse skeletal muscle lysate
Lane 8: Mouse brain lysate
Lane 9: Rat lung lysate
Lane 10: Rat skeletal muscle lysate
Lane11: Rat brain lysate