Anti-ENO1 Polyclonal Antibody (HY081014)

Name: Anti-ENO1 Polyclonal Antibody
Brand: abinScience
SKU: HY081014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY081014

説明

Anti-ENO1 Polyclonal Antibody (HY081014) is a rabbit polyclonal antibody detecting ENO1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Anas platyrhynchos, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human ENO1 (Pro267-Leu354).

ターゲット

Non-neural enolase, 2-phospho-D-glycerate hydro-lyase, MPB-1, ENO1, Plasminogen-binding protein, Enolase 1, C-myc promoter-binding protein, Alpha-enolase, MBP-1, MBPB1, MPB1, ENO1L1, NNE, Phosphopyruvate hydratase

精製

Purified by antigen affinity column.

アクセッション番号

P06733

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Alpha-enolase (ENO1) is a ~47 kDa protein. Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Also involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

1. Sugahara, T. et al. (1992) Cytotechnology 10, 137-46. PMID: 1369209

2. Huang, H. et al. (2018) Molecular cell 70, 663-678.e6. PMID: 29775581

3. Feo, S. et al. (2000) FEBS letters 473, 47-52. PMID: 10802057

4. López-Alemany, R. et al. (2003) American journal of hematology 72, 234-42. PMID: 12666133

5. Ray, R. et al. (1991) Molecular and cellular biology 11, 2154-61. PMID: 2005901

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-ENO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: LO2 cell lysate
Lane 3: PC-3 cell lysate
Lane 4: SGC-7901 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: Mouse lung lysate
Lane 7: Mouse skeletal muscle lysate
Lane 8: Mouse brain lysate
Lane 9: Rat lung lysate
Lane 10: Rat skeletal muscle lysate
Lane11: Rat brain lysate