Anti-ENO1 Polyclonal Antibody (HY081014)

Name: Anti-ENO1 Polyclonal Antibody
Brand: abinScience
SKU: HY081014
Price: 96 USD
Availability: InStock

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Каталожный номер

HY081014

Описание

Anti-ENO1 Polyclonal Antibody (HY081014) is a rabbit polyclonal antibody detecting ENO1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Anas platyrhynchos, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human ENO1 (Pro267-Leu354).

Цель

Non-neural enolase, 2-phospho-D-glycerate hydro-lyase, MPB-1, ENO1, Plasminogen-binding protein, Enolase 1, C-myc promoter-binding protein, Alpha-enolase, MBP-1, MBPB1, MPB1, ENO1L1, NNE, Phosphopyruvate hydratase

Очистка

Purified by antigen affinity column.

Номер доступа

P06733

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Alpha-enolase (ENO1) is a ~47 kDa protein. Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Also involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

1. Sugahara, T. et al. (1992) Cytotechnology 10, 137-46. PMID: 1369209

2. Huang, H. et al. (2018) Molecular cell 70, 663-678.e6. PMID: 29775581

3. Feo, S. et al. (2000) FEBS letters 473, 47-52. PMID: 10802057

4. López-Alemany, R. et al. (2003) American journal of hematology 72, 234-42. PMID: 12666133

5. Ray, R. et al. (1991) Molecular and cellular biology 11, 2154-61. PMID: 2005901

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-ENO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: LO2 cell lysate
Lane 3: PC-3 cell lysate
Lane 4: SGC-7901 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: Mouse lung lysate
Lane 7: Mouse skeletal muscle lysate
Lane 8: Mouse brain lysate
Lane 9: Rat lung lysate
Lane 10: Rat skeletal muscle lysate
Lane11: Rat brain lysate