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Anti-ENPP2 Polyclonal Antibody (HC423014)

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Aperçu
Numéro de catalogueHC423014
Description
Anti-ENPP2 Polyclonal Antibody (HC423014) is a rabbit polyclonal antibody detecting ENPP2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ENPP2 (Ser637-Ile863).
Cible LysoPLD, Extracellular lysophospholipase D, ENPP2, Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, PDNP2, Autotaxin, ATX
Purification Purified by antigen affinity column.
Numéro d'accès Q13822
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Autotaxin (ENPP2) is a ~98 kDa protein. Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Its major substrate is lysophosphatidylcholine. Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth.

1. Aoki, J. et al. (2002) The Journal of biological chemistry 277, 48737-44. PMID: 12354767
2. Clair, T. et al. (2003) Cancer research 63, 5446-53. PMID: 14500380
3. van Meeteren, LA. et al. (2005) The Journal of biological chemistry 280, 21155-61. PMID: 15769751
4. Stein, AJ. et al. (2015) Molecular pharmacology 88, 982-92. PMID: 26371182
5. Bain, G. et al. (2017) The Journal of pharmacology and experimental therapeutics 360, 1-13. PMID: 27754931
6. Tokumura, A. et al. (2002) The Journal of biological chemistry 277, 39436-42. PMID: 12176993
9. Bolen, AL. et al. (2011) Journal of lipid research 52, 958-70. PMID: 21393252
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ENPP2 Polyclonal Antibody

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