Anti-ERCC5 Polyclonal Antibody (HB668014)

Name: Anti-ERCC5 Polyclonal Antibody
Brand: abinScience
SKU: HB668014
Price: 168 USD
Availability: InStock

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Especificación:

50ug
100ug

Número:

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Número de catálogo

HB668014

Descripción

Anti-ERCC5 Polyclonal Antibody (HB668014) is a rabbit polyclonal antibody detecting ERCC5 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human ERCC5 (Leu778-Ala986).

Objetivo

ERCC5, DNA excision repair protein ERCC-5, ERCM2, XPGC, Xeroderma pigmentosum group G-complementing protein, XPG, DNA repair protein complementing XP-G cells

Purificación

Purified by antigen affinity column.

Número de acceso

P28715

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

DNA excision repair protein ERCC-5 (ERCC5) is a ~133 kDa protein. Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes.

1. Tsutakawa, SE. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 14127-14138. PMID: 32522879

2. González-Corrochano, R. et al. (2020) Nucleic acids research 48, 9943-9958. PMID: 32821917

3. Cloud, KG. et al. (1995) Mutation research 347, 55-60. PMID: 7651464

4. Habraken, Y. et al. (1994) Nucleic acids research 22, 3312-6. PMID: 8078765

5. O'Donovan, A. et al. (1994) Nature 371, 432-5. PMID: 8090225

6. O'Donovan, A. et al. (1994) The Journal of biological chemistry 269, 15965-8. PMID: 8206890

7. Bessho, T. (1999) Nucleic acids research 27, 979-83. PMID: 9927729

8. Sarker, AH. et al. (2005) Molecular cell 20, 187-98. PMID: 16246722

10. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-ERCC5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-ERCC5 Polyclonal Antibody (HB668014)

Vista general

Imágenes

Referencias

Datasheet

SDS