Human ERCC5 Recombinant Protein (N-His) (HB668012)

Name: Human ERCC5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB668012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB668012
Description	Recombinant Human ERCC5 Protein, N-His (HB668012) expressed in E. coli, spanning Leu778-Ala986. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P28715
Protein length	Leu778-Ala986
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	26.37 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ERCC5, DNA excision repair protein ERCC-5, ERCM2, XPGC, Xeroderma pigmentosum group G-complementing protein, XPG, DNA repair protein complementing XP-G cells
Background	DNA excision repair protein ERCC-5 (ERCC5) is a ~133 kDa protein. Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. 1. Tsutakawa, SE. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 14127-14138. PMID: 32522879 2. González-Corrochano, R. et al. (2020) Nucleic acids research 48, 9943-9958. PMID: 32821917 3. Cloud, KG. et al. (1995) Mutation research 347, 55-60. PMID: 7651464 4. Habraken, Y. et al. (1994) Nucleic acids research 22, 3312-6. PMID: 8078765 5. O'Donovan, A. et al. (1994) Nature 371, 432-5. PMID: 8090225 6. O'Donovan, A. et al. (1994) The Journal of biological chemistry 269, 15965-8. PMID: 8206890 7. Bessho, T. (1999) Nucleic acids research 27, 979-83. PMID: 9927729 8. Sarker, AH. et al. (2005) Molecular cell 20, 187-98. PMID: 16246722 10. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769
Note	For research use only.

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SDS-PAGE for Recombinant Human ERCC5 Protein, N-His

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Datasheet

Human ERCC5 Recombinant Protein (N-His).pdf

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Human ERCC5 Recombinant Protein (N-His) (HB668012)

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