Anti-ERCC5 Polyclonal Antibody (HB668014)

Name: Anti-ERCC5 Polyclonal Antibody
Brand: abinScience
SKU: HB668014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HB668014

Description

Anti-ERCC5 Polyclonal Antibody (HB668014) is a rabbit polyclonal antibody detecting ERCC5 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ERCC5 (Leu778-Ala986).

Cible

ERCC5, DNA excision repair protein ERCC-5, ERCM2, XPGC, Xeroderma pigmentosum group G-complementing protein, XPG, DNA repair protein complementing XP-G cells

Purification

Purified by antigen affinity column.

Numéro d'accès

P28715

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

DNA excision repair protein ERCC-5 (ERCC5) is a ~133 kDa protein. Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes.

1. Tsutakawa, SE. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 14127-14138. PMID: 32522879

2. González-Corrochano, R. et al. (2020) Nucleic acids research 48, 9943-9958. PMID: 32821917

3. Cloud, KG. et al. (1995) Mutation research 347, 55-60. PMID: 7651464

4. Habraken, Y. et al. (1994) Nucleic acids research 22, 3312-6. PMID: 8078765

5. O'Donovan, A. et al. (1994) Nature 371, 432-5. PMID: 8090225

6. O'Donovan, A. et al. (1994) The Journal of biological chemistry 269, 15965-8. PMID: 8206890

7. Bessho, T. (1999) Nucleic acids research 27, 979-83. PMID: 9927729

8. Sarker, AH. et al. (2005) Molecular cell 20, 187-98. PMID: 16246722

10. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769

Note

For research use only.

Images

Reférences

Fiche technique

Anti-ERCC5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-ERCC5 Polyclonal Antibody (HB668014)

Aperçu

Images

Reférences

Fiche technique

SDS